Effects of Hydro-Methanolic Extract of Macadamia Nuts on Haematological Parameters in Male Wistar Rats
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Abstract
Background: To evaluate the effects of the hydro-methanolic extract of Macadamia integrifolia nuts on haematological parameters in male Wistar rats.
Methods: The study employed an experimental, controlled laboratory study using a dose–dependent treatment model. The study was conducted in an animal house of the Department of Human Physiology, Rivers State University, Nigeria. Twenty-four male Wistar rats were randomly assigned into four groups (n=6 per group): control, low dose (250 mg/kg), medium dose (500 mg/kg), and high dose (1000 mg/kg) of hydro-methanolic Macadamia integrifolia nuts extract. The extract was administered daily for 28 days. Wistar rats received daily administration of hydro-methanolic extract of Macadamia integrifolia nuts for 28 days. Standard haematological parameters were analysed using established laboratory methods. Data were expressed as mean ± SEM, and statistical significance was set at P < 0.05.
Results: Significant dose-dependent alterations were observed in erythrocyte indices. The low-dose group showed marked reductions in packed cell volume (33.0 ± 0.6% vs 45.3 ± 1.5%), haemoglobin (11.0 ± 0.2 g/dL vs 15.1 ± 0.5 g/dL), and red blood cell count (5.0 ± 0.3 ×10⁶/µL vs 6.7 ± 0.1 ×10⁶/µL) compared with control (P < 0.05). The medium dose partially attenuated these reductions. In contrast, the high dose restored packed cell volume (44.3 ± 1.5%), haemoglobin (14.97 ± 0.7 g/dL), and red blood cell count (6.02 ± 0.1 ×10⁶/µL) to values comparable with control. Mean corpuscular haemoglobin increased significantly at low and medium doses, while mean corpuscular haemoglobin concentration remained unchanged across groups. Mean corpuscular volume decreased at low and medium doses but increased at the high dose. White blood cell count was significantly elevated at the high dose (13.57 ± 1.1 ×10³/µL) relative to control (10.8 ± 1.0 ×10³/µL; P < 0.05), whereas platelet count and differential leukocyte percentages showed no significant differences.
Conclusion: Hydro-methanolic Macadamia integrifolia nuts extract exerted biphasic, dose-dependent effects on haematological parameters, with low doses associated with erythrocyte suppression and higher doses restoring erythroid indices and increasing total leukocyte count.
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References
I. Aune, D., Keum, N., Giovannucci, E., Fadnes, L. T., Boffetta, P., Greenwood, D. C., Tonstad, S., Vatten, L. J., Riboli, E., & Norat, T. (2016). Nut consumption and risk of cardiovascular disease, total cancer, all-cause and cause-specific mortality: a systematic review and dose-response meta-analysis of prospective studies. BMC Med, 14(1), 207. https://doi.org/10.1186/s12916-016-0730-3
II. Batubo, N. P., Ogbu, O. S., & Victor, D. D. (2023). Hematological, histopathological and oxidative stress responses to n-hexane extract of Terminalia catappa nuts in leukemia-induced Wistar rats. International Journal of Research in Medical Sciences, 12(1), 61-
https://doi.org/10.18203/2320-6012.ijrms20233978
III. Batubo, N. P., Ogbu, O. S., & Victor, D. D.;. (2023). Chemical profiles and proximate analysis of n-hexane extract of Terminalia catappa kernel from Nigeria. International Journal of Research in Medical Sciences, 12(1), 17-25. https://doi.org/10.18203/2320-6012.ijrms20233971
IV. Calabrese, E. J., & Kozumbo, W. J. (2021). The hormetic dose-response mechanism: Nrf2 activation. Pharmacol Res, 167, 105526. https://doi.org/10.1016/j.phrs.2021.105526
V. Calder, P. C. (2020). Nutrition, immunity and COVID-19. BMJ Nutr Prev Health, 3(1), 74-92. https://doi.org/10.1136/bmjnph-2020-000085
VI. Camaschella, C. (2019). Iron deficiency. Blood, 133(1), 30-39. https://doi.org/10.1182/blood-2018-05-815944
VII. Capece, U., Gugliandolo, S., Morciano, C., Avolio, A., Splendore, A., Di Giuseppe, G., Ciccarelli, G., Soldovieri, L., Brunetti, M., Mezza, T., Pontecorvi, A., Giaccari, A., & Cinti, F. (2024). Erythrocyte Membrane Fluidity and Omega-3 Fatty Acid Intake: Current Outlook and Perspectives for a Novel, Nutritionally Modifiable Cardiovascular Risk Factor. Nutrients, 16(24). https://doi.org/10.3390/nu16244318
VIII. Delimont, N. M., Haub, M. D., & Lindshield, B. L. (2017). The Impact of Tannin Consumption on Iron Bioavailability and Status: A Narrative Review. Curr Dev Nutr, 1(2), 1-12. https://doi.org/10.3945/cdn.116.000042
IX. Furman, D., Campisi, J., Verdin, E., Carrera-Bastos, P., Targ, S., Franceschi, C., Ferrucci, L., Gilroy, D. W., Fasano, A., Miller, G. W., Miller, A. H., Mantovani, A., Weyand, C. M., Barzilai, N., Goronzy, J. J., Rando, T. A., Effros, R. B., Lucia, A., Kleinstreuer, N., & Slavich, G. M. (2019). Chronic inflammation in the etiology of disease across the life span. Nat Med, 25(12), 1822-1832. https://doi.org/10.1038/s41591-019-0675-0
X. Ganesan, K., & Xu, B. (2017). Polyphenol-Rich Dry Common Beans (Phaseolus vulgaris L.) and Their Health Benefits. Int J Mol Sci, 18(11). https://doi.org/10.3390/ijms18112331
XI. Glenn, A. J., Aune, D., Freisling, H., Mohammadifard, N., Kendall, C. W. C., Salas-Salvado, J., Jenkins, D. J. A., Hu, F. B., & Sievenpiper, J. L. (2023). Nuts and Cardiovascular Disease Outcomes: A Review of the Evidence and Future Directions. Nutrients, 15(4). https://doi.org/10.3390/nu15040911
XII. Gulcin, I. (2025). Antioxidants: a comprehensive review. Arch Toxicol, 99(5), 1893-1997. https://doi.org/10.1007/s00204-025-03997-2
XIII. Hung, H. C., Tsai, S. F., Chou, H. W., Tsai, M. J., Hsu, P. L., & Kuo, Y. M. (2023). Dietary fatty acids differentially affect secretion of pro-inflammatory cytokines in human THP-1 monocytes. Sci Rep, 13(1), 5511. https://doi.org/10.1038/s41598-023-32710-5
XIV. Kaushansky, K. (2015). Thrombopoiesis. Semin Hematol, 52(1), 4-11. https://doi.org/10.1053/j.seminhematol.2014.10.003
XV. Lorenzon Dos Santos, J., Quadros, A. S., Weschenfelder, C., Garofallo, S. B., & Marcadenti, A. (2020). Oxidative Stress Biomarkers, Nut-Related Antioxidants, and Cardiovascular Disease. Nutrients, 12(3). https://doi.org/10.3390/nu12030682
XVI. National Institutes of Health. (2011). National Research Council (US) Committee for the Update of the Guide for the Care and Use of Laboratory Animals. In Guide for the Care and Use of Laboratory Animals (8th ed.). https://doi.org/10.17226/12910
XVII. Obeagu, E. I., Igwe, M. C., & Obeagu, G. U. (2024). Oxidative stress's impact on red blood cells: Unveiling implications for health and disease. Medicine (Baltimore), 103(9), e37360. https://doi.org/10.1097/MD.0000000000037360
XVIII. OECD. (2002). Test No. 423: Acute Oral toxicity - Acute Toxic Class Method, OECD Guidelines for the Testing of Chemicals. P. OECD Publishing.
XIX. Park, M., & Chang, Y. H. (2025). Clinical Significance of Red Blood Cell Indices. In V. Rajashekaraiah (Ed.), Red Blood Cells - Functions and Significance. IntechOpen. https://doi.org/10.5772/intechopen.1008323
XX. Pretorius, E., Olumuyiwa-Akeredolu, O. O., Mbotwe, S., & Bester, J. (2016). Erythrocytes and their role as health indicator: Using structure in a patient-orientated precision medicine approach. Blood Rev, 30(4), 263-274. https://doi.org/10.1016/j.blre.2016.01.001
XXI. R Core Team. (2024). _R: A Language and Environment for Statistical Computing. R Foundation for Statistical Computing. In (Version 4.4.2) Vienna, Austria. https://www.R-project.org/.
XXII. Rosales, C. (2018). Neutrophil: A Cell with Many Roles in Inflammation or Several Cell Types? Front Physiol, 9, 113. https://doi.org/10.3389/fphys.2018.00113
XXIII. Schwingshackl, L., Schwedhelm, C., Hoffmann, G., Knuppel, S., Iqbal, K., Andriolo, V., Bechthold, A., Schlesinger, S., & Boeing, H. (2017). Food Groups and Risk of Hypertension: A Systematic Review and Dose-Response Meta-Analysis of Prospective Studies. Adv Nutr, 8(6), 793-803. https://doi.org/10.3945/an.117.017178
XXIV. Seham El-Hawary, M. A., Engy A. Mahrous, . (2022). Extracts of different organs of Macadamia integrifolia ameliorate oxidative damage in a D‑galactose accelerated ageing model in rats. . Biointerface Research in Applied Chemistry, 12(5), 7125-7135. https://doi.org/https://doi.org/10.33263/BRIAC125.71257135