Effect of Hydro-Methanolic Extract of Macadamia Integrifolia Nuts on Oxidative Stress and Inflammatory Markers in Male Wistar Rats
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Abstract
Background: Oxidative stress and inflammation contribute to the pathogenesis of several chronic diseases. Bioactive compounds in tree nuts, including Macadamia integrifolia, may influence redox balance and inflammatory signalling. This study evaluated the effects of hydro-methanolic Macadamia integrifolia nut extract on oxidative stress and inflammatory markers in male Wistar rats.
Methods: Twenty-four male Wistar rats were randomly allocated into four groups (n = 6 per group): control, 250 mg/kg, 500 mg/kg, and 1000 mg/kg extract-treated groups. The extract was administered orally once daily for 28 days. At study completion, serum levels of catalase (CAT), superoxide dismutase (SOD), malondialdehyde (MDA), glutathione (GSH), glutathione peroxidase (GPx), tumour necrosis factor-alpha (TNF-α), interleukin-6 (IL-6), C-reactive protein (CRP), interleukin-10 (IL-10), and nuclear factor kappa B (NF-κB) were measured using standard spectrophotometric and ELISA methods. Statistical analysis was performed using one-way ANOVA with Tukey’s post hoc test.
Results: CAT and SOD activities showed no significant differences across all groups (p > 0.05). MDA levels were also comparable between control and treated groups, indicating no significant change in lipid peroxidation. In contrast, GSH levels decreased progressively with increasing extract dose, with significant reductions observed in the 500 mg/kg and 1000 mg/kg groups compared with the control (p < 0.05). GPx activity followed a similar dose-dependent decline, with significant reductions in the medium- and high-dose groups (p < 0.05). For inflammatory markers, TNF-α and IL-6 concentrations did not differ significantly among groups (p > 0.05). However, CRP levels were significantly lower in the 500 mg/kg and 1000 mg/kg groups compared with the control (p < 0.05). IL-10 and NF-κB concentrations also decreased significantly in the medium- and high-dose groups (p < 0.05), demonstrating a dose-dependent modulation of inflammatory signalling pathways.
Conclusion: Hydro-methanolic Macadamia integrifolia extract exerted selective, dose-dependent effects on glutathione-related antioxidant systems and inflammatory biomarkers without altering primary enzymatic antioxidants or lipid peroxidation.
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References
I. Altanam, S. Y., Darwish, N., & Bakillah, A. (2025). Exploring the Interplay of Antioxidants, Inflammation, and Oxidative Stress: Mechanisms, Therapeutic Potential, and Clinical Implications. Diseases, 13(9).https://doi.org/10.3390/diseases13090309
II. Andres, C. M. C., Perez de la Lastra, J. M., Juan, C. A., Plou, F. J., & Perez-Lebena, E. (2024). Antioxidant Metabolism Pathways in Vitamins, Polyphenols, and Selenium: Parallels and Divergences. Int J Mol Sci, 25(5).https://doi.org/10.3390/ijms25052600
III. Camacho-Teodocio, J. D., Gallardo-Velázquez, T., Osorio-Revilla, G., Castañeda-Pérez, E., Velázquez-Contreras, C., Cornejo-Mazón, M., & Hernández-Martínez, D. M. (2024). Macadamia (Macadamia integrifolia) Nut-Based Beverage: Physicochemical Stability and Nutritional and Antioxidant Properties. Beverages, 10(3), 58. https://www.mdpi.com/2306-5710/10/3/58
IV. Dubois-Deruy, E., Peugnet, V., Turkieh, A., & Pinet, F. (2020). Oxidative Stress in Cardiovascular Diseases. Antioxidants (Basel), 9(9).https://doi.org/10.3390/antiox9090864
V. Houston, L., Probst, Y. C., Chandra Singh, M., & Neale, E. P. (2023). Tree Nut and Peanut Consumption and Risk of Cardiovascular Disease: A Systematic Review and Meta-Analysis of Randomized Controlled Trials. Adv Nutr, 14(5), 1029-1049. https://doi.org/10.1016/j.advnut.2023.05.004
VI. Hung, H. C., Tsai, S. F., Chou, H. W., Tsai, M. J., Hsu, P. L., & Kuo, Y. M. (2023). Dietary fatty acids differentially affect secretion of pro-inflammatory cytokines in human THP-1 monocytes. Sci Rep, 13(1), 5511. https://doi.org/10.1038/s41598-023-32710-5
VII. Jiang, R., Jacobs, D. R., Jr., Mayer-Davis, E., Szklo, M., Herrington, D., Jenny, N. S., Kronmal, R., & Barr, R. G. (2006). Nut and seed consumption and inflammatory markers in the multi-ethnic study of atherosclerosis. Am J Epidemiol, 163(3), 222-231. https://doi.org/10.1093/aje/kwj033
VIII. Jiang, S., Liu, H., & Li, C. (2021). Dietary Regulation of Oxidative Stress in Chronic Metabolic Diseases. Foods, 10(8). https://doi.org/10.3390/foods10081854
IX. Kalogerakou, T., & Antoniadou, M. (2024). The Role of Dietary Antioxidants, Food Supplements and Functional Foods for Energy Enhancement in Healthcare Professionals. Antioxidants (Basel), 13(12). https://doi.org/10.3390/antiox13121508
X. Lawrence, T. (2009). The nuclear factor NF-kappaB pathway in inflammation. Cold Spring Harb Perspect Biol, 1(6), a001651. https://doi.org/10.1101/cshperspect.a001651
XI. Mititelu, M., Lupuliasa, D., Neacsu, S. M., Olteanu, G., Busnatu, S. S., Mihai, A., Popovici, V., Maru, N., Boroghina, S. C., Mihai, S., Ionita-Mindrican, C. B., & Scafa-Udriste, A. (2024). Polyunsaturated Fatty Acids and Human Health: A Key to Modern Nutritional Balance in Association with Polyphenolic Compounds from Food Sources. Foods, 14(1). https://doi.org/10.3390/foods14010046
XII. National Institutes of Health. (2011). National Research Council (US) Committee for the Update of the Guide for the Care and Use of Laboratory Animals. In Guide for the Care and Use of Laboratory Animals (8th ed.). https://doi.org/10.17226/12910
XIII. OECD. (2002). Test No. 423: Acute Oral toxicity - Acute Toxic Class Method, OECD Guidelines for the Testing of Chemicals. P. OECD Publishing.
XIV. Platzer, M., Kiese, S., Tybussek, T., Herfellner, T., Schneider, F., Schweiggert-Weisz, U., & Eisner, P. (2022). Radical Scavenging Mechanisms of Phenolic Compounds: A Quantitative Structure-Property Relationship (QSPR) Study. Front Nutr, 9, 882458. https://doi.org/10.3389/fnut.2022.882458
XV. R Core Team. (2024). _R: A Language and Environment for Statistical Computing. R Foundation for Statistical Computing. In (Version 4.4.2) Vienna, Austria. https://www.R-project.org/.
XVI. Rajaram, S., Damasceno, N. R. T., Braga, R. A. M., Martinez, R., Kris-Etherton, P., & Sala-Vila, A. (2023). Effect of Nuts on Markers of Inflammation and Oxidative Stress: A Narrative Review. Nutrients, 15(5). https://doi.org/10.3390/nu15051099
XVII. Ros, E. (2010). Health benefits of nut consumption. Nutrients, 2(7), 652-682. https://doi.org/10.3390/nu2070652
XVIII. Santa-Maria, C., Lopez-Enriquez, S., Montserrat-de la Paz, S., Geniz, I., Reyes-Quiroz, M. E., Moreno, M., Palomares, F., Sobrino, F., & Alba, G. (2023). Update on Anti-Inflammatory Molecular Mechanisms Induced by Oleic Acid. Nutrients, 15(1). https://doi.org/10.3390/nu15010224
XIX. Saraiva, M., & O'Garra, A. (2010). The regulation of IL-10 production by immune cells. Nat Rev Immunol, 10(3), 170-181.https://doi.org/10.1038/nri2711
XX. Seham El-Hawary, M. A., Engy A. Mahrous, . (2022). Extracts of different organs of Macadamia integrifolia ameliorate oxidative damage in a D‑galactose accelerated ageing model in rats. . Biointerface Research in Applied Chemistry, 12(5), 7125-7135. https://doi.org/https://doi.org/10.33263/BRIAC125.71257135
XXI. Sies, H., Berndt, C., & Jones, D. P. (2017). Oxidative Stress. Annu Rev Biochem, 86, 715-748. https://doi.org/10.1146/annurev-biochem-061516-045037
XXII. Tu, X. H., Wu, B. F., Xie, Y., Xu, S. L., Wu, Z. Y., Lv, X., Wei, F., Du, L. Q., & Chen, H. (2021). A comprehensive study of raw and roasted macadamia nuts: Lipid profile, physicochemical, nutritional, and sensory properties. Food Sci Nutr, 9(3), 1688-1697. https://doi.org/10.1002/fsn3.2143
XXIII. Yilgor, A., & Demir, C. (2024). Determination of oxidative stress level and some antioxidant activities in refractory epilepsy patients. Sci Rep, 14(1), 6688. https://doi.org/10.1038/s41598-024-57224-6
XXIV. Zhang, M., Zhang, Y., Li, L., Wei, C., Dai, T., Li, Y., Shuai, X., & Du, L. (2024). Macadamia (Macadamia integrifolia) Oil Prevents High-Fat Diet-Induced Lipid Accumulation and Oxidative Stress by Activating the AMPK/Nrf2 Pathway. Foods, 13(22).