Characterization of Bioactive Chemical Compounds from Staphylococcus aureus and Evaluation of Antibacterial Activity

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Published: Nov 15, 2023
DOI: https://doi.org/10.47191/ijpbms/v3-i11-10
Keywords:
Staphylococcus aureus , Secondary metabolites, Antibacterial, GC/MS

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Rabab J.H. Al-Hasseny
Medical Microbiology, Department of Health Food and Nutrition, College of Food Science,Al-Qasim Green University, Iraq
Ahmed Obaid Hossain
College of Biotechnology, Al-Qasim Green University, Iraq.
Abbas K. Al-Mansoori
Department of Genetic Engineering, Faculty of Biotechnology, Al-Qasim Green University, Iraq.

Abstract





Aims and Objectives:  This research aimed to analyze the bioactive chemical products of Staphylococcus aureus and evaluate the antibacterial and in vitro antimicrobial activities of plant extracts against Staphylococcus aureus.


Method: The chemical components known as bioactives, which are sometimes referred to as secondary metabolites, were examined using gas chromatography-mass spectrometry (GC-MS) techniques. Subsequently, the antibacterial activity of the methanolic extract of Staphylococcus aureus was assessed in vitro.


Results: The GC-MS analysis of Staphylococcus aureus detected the presence of the following:   The compounds listed include ethyl 12-aminododecanoate, 1,11-Diaminoundecan-6-ol, N-propylidenehydroxylamine, 3,5-diamino-2,6-dicyanopyrazine, and methyl 1-methylpiperidine-3-carboxylate.   The compound is called 5-azoniaspiro[4.5]decane.   The compounds mentioned include 3,3-Dimethyl-2-acetyloxirane, 2-Hexadecanol, and 2-methyl-2-hexadecanol.   The compounds mentioned are 12,15-octadecadiynoic acid and 9,11-octadecadiynoic acid.   8-oxo-   2-Ethyl-3,5-dimethylpyridine, 3,5-Dimethyl-2-ethylpyridine, ethyl 5,6-dimethylpyridine-3-carboxylate, 10-Oxododecanoic acid, and 12-Ethoxy-12-oxododecanoic acid.   Glycyl-D-asparagine is a compound.   2,5-Piperazinedione, 3,6-bis(2-methylpropyl)-1H-pyrazin-2-one, and l-Leucyl-d-leucine. The evaluation of the antibacterial activity revealed that the metabolites of Staphylococcus aureus had a remarkably high level of activity against Escherichia coli (9.28±0.05).  Equisetum arvense extract exhibited significant antimicrobial activity against Staphylococcus aureus.





Article Details

How to Cite
Rabab J.H. Al-Hasseny, Ahmed Obaid Hossain, & Abbas K. Al-Mansoori. (2023). Characterization of Bioactive Chemical Compounds from Staphylococcus aureus and Evaluation of Antibacterial Activity. International Journal of Pharmaceutical and Bio Medical Science, 3(11). https://doi.org/10.47191/ijpbms/v3-i11-10
Issue
Vol. 3 No. 11 (2023): Volume 03 Issue 11 November 2023
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Articles
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This work is licensed under a Creative Commons Attribution 4.0 International License.

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