The Effect of Broccoli Extract (Brassica Oleracea Var.) on the Kidney Glomerulus of Male Wistar Rats (Rattus Norvegicus) Induced by Alloxan
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Abstract
Aloxan causes an imbalance between antioxidants and free radicals, leading to health implications including kidney dysfunction, which can be observed, among other factors, by the extent of the kidney glomerulus area. Exogenous antioxidant intake is needed to prevent a decrease in the glomerulus area due to free radicals induced by aloxan, one of which is broccoli (Brassica oleracea var. italica) which has high levels of antioxidants. Objective: This study aims to determine the effect of broccoli extract (Brassica oleracea var. italica) on the changes in the glomerulus area of male Wistar rats induced by aloxan. Methodology: This research used an experimental method, namely RCT (Randomized Controlled Trial), with a post-test control group design on 32 male Wistar rats for 30 days, divided into 4 groups: negative control group, positive control group, and 2 treatment groups (100 mg/kgBW and 200 mg/kgBW). The calculation of the glomerulus area in the kidneys was performed with a magnification of 400x. The data obtained will be processed using ANOVA test to observe differences between groups. Research Results: This study showed differences in the glomerulus area of the kidneys in all groups (ANOVA, p=0.00). The increase in broccoli extract administration is directly proportional to the glomerulus area in the group given aloxan and yielded significant results (LSD, p<0.05). Conclusion: Administration of broccoli extract can increase the glomerulus area of Wistar rat kidneys induced by aloxan.
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