Antioxidant Activity Test of Outstreched Bark Extract (Campnosperma Brevipetiolatum Volkens) using the DPPH Method
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Abstract
Outstretched bark (Campnosperma brevipetiolatum Volken) is rich in tannins, flavonoids, alkaloids, and saponins. Herbal plants contain flavonoids, which are excellent antioxidants. Determine the amounts of phytochemical substances present in the sample and assess the antioxidant activity using the DPPH (1.1-diphenyl, 2-picrylhydrazil) technique at concentrations of 50, 100, 150, 200, 250, and 300 parts per million. The presenance of alkaloids alkaloids, saponins, and tannins was detected in the phytochemical screening findings against ethanol extracts of outstretched bark. 96% ethanol solvent is used in the maceration technique of extraction. Vitamin C is the positive control utilized. According to the findings of tests for antioxidant activity, ethanol extract at 11.25 ppm has a very high potential for antioxidant activity because its IC50 value is 3.55 ppm.
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References
I. Winarsi H. 2007. Antioksidan alami dan Radikal Bebas. Yogyakarta: Kanisius.
II. Behere, D. R. 2013. “Medicinal Orchids in India and Their Conversation” 7(1): 5359. http://www.globalsciencebooks.info/Online/GSBOnline/images/2013/F OB_7(1)/FOB_7(1) 53-59o.pdf Cronquist, Artur. 1981. An integrated system of classification of flowering plants. The New York Botanical Garden: Columbia University Press.
III. Badarinath, A., Rao, K., Chetty, C. S, Ramkanth, S., Rajan, T., & Gnanaprakash, K.A., (2010). A Review on In-vitro Antioxidant Methods: Comparisons, Correlations, and Considerations. International Journal of PharmTech Research, 5, 1276-1285. Brown, L. D., Hua, H., and Gao, C. 2003. A widget framework for augmented interaction in SCAPE.
IV. Mandal S, Yadav S, Nema R., (2009). Antioxidants: A review. Journal of Chemical and Pharmaceutical Research, 102-104.
V. Brand- Williams, W., CUvelier, M., and Berset, C., (1995). Use of a free radical method to evaluate antioxidant activity. LWT-Food Science and Technology, 28(1), 25–30.
VI. Simanjuntak K. (2012). Peran Antibiotik Dalam Meningkatkan Kesehatan. Vol. 23(3), 135-140.
VII. Yunita Arian Sani Anwar, 2017. Aktivitas Antioksidan Ekstrak Ampas Buah Semu Jambu Mete ((anacardium occidentale linn) dan Pengaruhnya Pada Pengolahan Minyak Kelapa Tradisional. Jurnal Penelitian Kimia, 13.
VIII. Herman Saud, 2021. Wawancara Pribadi “Tumbuhan Terentang”. Jayapura.
IX. Anggraini Dewi, 2010. Perancangan Komunikasi Virtual Kemasan Nuslik PT. Pusaka Tradisi Ibu. Skripsi. Jakarta: BINUS
X. Ahmaad T, Singh S.B & Pandey S. 2013. Phytochemical Screening and Physicochemical Parameters of Crude Drugs: A Brief Review. International Journal of Pharma Research and Review, 2, 53-60.
XI. Tiwari, P., Kumar, B., Kaur, M., Kaur G. & Kaur H., 2011, Phytochemical Screening and Extraction: A Review, International Pharmaceutica Sciencia, 1(1), 98-106
XII. Molyneux, P. (2004). The Use of The Sable Free Radical Diphenylpicrylhydrazyl (DPPH) for Estimating Antioxidant Activity SongklanarinJ. Sci. Technol. Morales-Gonzales. (2003). Oxidative Stress and Chronic Degenerative Diseases: a Role for Antioxidants. Croatia: Intech Publisher.
XIII. Waji, R.A, dan Sugrani, A. (2009). Flavonoid (Quercetin). Kimia Organik Bahan Alam. Program S2 Kimia. Fakultas Matematika dan Ilmu Pengetahuan Alam. Universitas Hasanuddin
XIV. McMurry, J. and R.C. Fay. (2004). McMurry Fay Chemistry. 4th edition. Belmont, CA.: Pearson Education International.
XV. Marliana, E. 2012. Akivitas Antioksidan Ekstrak Etanol Daun Andong (Cordyline frusticosa [L]. A Cheval), Mulawaman Scientifile, Vol. 11 (1), 71-81
XVI. Sangi, M., et al. 2008. Analisis Fiokimia Tumbuhan Obat di Kabupaten Minahasa Utara. Chemistry Progress. 1, 47-53.
XVII. Dewi, Made Yustiari dan I Ketut Sujana. 2014. “Pengaruh Ukuran Perusahaan dan Profitabilitas Pada Praktik Perataan Laba Dengan Jenis Industri Sebagai Variabel Pemoderasi Di BEI”. ISSN 2302-8556. E-journal Akuntansi Universitas Udayana. 170-184. Bali.
XVIII. Dehpour, A. A., Ebrahimzadeh, M. A., Fazel, N. S. & Mohammad, N. S., 2009, Antioxidant activity of the methanol extract of Ferula assafoetida and its essential oil composition, Grasas Aceites, 60 (4).
XIX. Vasic, S.M., Stefanovic, O.D., Licina, B.Z.,Radojevic, I.D., Comic, L.R. (2012). Biological Activities of Extracts from Cultivated Granadilla Passiflora alata. EXCLI Journal. Vol 11 ISSN 1611-2156
XX. Gurav, S., Nilambari, D., Vijay, G., Nandkishore, D., & Arun, P. 2007. Free Radical Scavenging Activity of Polygala Chinensis Linn. Pharmacologyonline. 2:245-253.
XXI. Aurora, A, M.G. Nair, and G.M Strasburg. 1998. Structure-Activity Relationships for Antioxidant Activities of a Series of Flavonoids In A Liposomal System. Free Radic. Biol. & Med. 24(9):1355-1363.
XXII. Sumardika, J. 2012. Water Extract of Sweet Potato Leaf Improved Lipid Profile and Blood SOD Content of Rats with High Cholesterol Diet. Medicina. 43(2).