HPTLC Fingerprint Profile and Standardization of Plantago ovata Forssk.
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Abstract
Plantago ovata belongs to the family Plantaginaceae and has notable medicinal properties as it is used for curing diarrhoea, dysentery and constipation due to its property of absorbing and retaining high amount of water. Methanolic extracts of P. ovata were obtained through reflux extraction to perform further experiments. Various experimental tests were carried out to understand the physicochemical, antimicrobial and fluorescence properties. HPTLC and phytochemical profiles were also obtained. Preliminary phytochemical analysis showed the presence of alkaloids, saponins, steroids and triterpenes. Microscopy of the powdered sample revealed the presence of starch granules, trichome and mucilage. The maximum antimicrobial activity of P. ovata was seen at a sample concentration of 10 µL where the activity was found to be more than standard against Bacillus cereus and Aspergillus niger. HPTLC profiling showed 9 bands each in 5 lanes of increasing concentration and a pattern unique to Plantago ovata.
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References
I. Anonymous, Wealth of Asia- CD, 1996, Executive Director, Asian Health, Environmental & Allied Databases (AHEAD), NISCOM (CSIR)
II. Medicinal Plants of the World, vol. 3: Chemical Constituents, Traditional and Modern Medicinal Uses By: I. A. Ross © Humana Press Inc., Totowa, NJ
III. Niinistö, K.E.; Ruohoniemi, M.O.; Freccero, F.; Raekallio, M.R, 2018,” Investigation of the treatment of sand accumulations in the equine large colon with psyllium and magnesium sulphate”, The Veterinary Journal. 238: 22–26. doi:10.1016/j.tvjl.2018.06.005
IV. Khalili, B.; Bardana, EJ.; Yunginger, JW, 2003, "Psyllium-associated anaphylaxis and death: a case report and review of the literature". Ann Allergy Asthma Immunol. 91 (6): 579–84. doi:10.1016/S1081-1206(10)61538-4
V. Alemán AM, Quirce S, Bombín C, Sastre J, 2001, "Asthma related to inhalation of Plantago ovata". Med Clin (Barc) (in Spanish). 116 (1): 20–2. doi:10.1016/S0025-7753(01)71705-8
VI. Clark, Cain; Salek, Mina; Aghabagheri, Elahe; Jafarnejad, Sadegh, 2019, "The effect of psyllium supplementation on blood pressure: a systematic review and meta-analysis of randomized controlled trials", Korean Journal of International Medicine. 35 (6): 1385–1399. doi:10.3904/kjim.2019.049
VII. Plantago ovata Forssk., “Flora of Presidency of Madras”, J. S. Gamble (1928), Vol. II, pp. 1159- 1160
VIII. A handbook of pharmacognosy by V.E, Tyler
IX. Evaluation of crude drugs, mono or polyherbal formulation www.pharmatutor.org/.../evaluation of crude drugs
X. Raghunathan. Pharmacopoeia Standards for Ayurvedic Formulations. Central Council for Research in Indian Medicine and Homeopathy, E-25, Defence Colony, New Delhi, 1976.
XI. Shome U: Pharmacognostic studies on Atemisia scoparia Waldst and Kit. Proceedings of the Indian Academy of Sciences - Section A Part 3 Mathematical Sciences 1984; 93(2): 151-164
XII. Gibbs RD: Chemotaxonomy of flowering plants, Mcgill Queen's University Press, Montreas 1974; 1: 523-619.
XIII. Kleipool RJC: Constituents of Andrographis paniculata. Nature, London, 1952; 169: 338.
XIV. Peach K and Tracey MV: Modern methods of plant analysis. By Springer-Verlag Ohg, Berlin. Heidelberg, New York, Published by Narosa Publishing House, New Delhi. Vol. II. 467-474, Vol. III, 1959: 586-589.
XV. Stahl E, 1965, “Thin layer chromatography”, Springer International Student Edition New York, 1965.10.
XVI. Johansen DA. Plant Microtechnique. McGraw-Hill, New York, 1940, 523
XVII. Chase Jr, C.R., Pratt R, 1949, “Fluorescence of powdered vegetable drugs with particular reference to development of a system of identification”, Journal of the American Pharmaceutical Association, 38(6), 324- 331.
XVIII. Indian drug Manufacturers’ (2002) Indian herbal pharmacopoeia, Indian drug Manufacturers’ Mumbai
XIX. WHO (1998c). Quality control methods for medicinal plant materials, WHO, Geneva. pp:28.
XX. Bauer, A.W., Kirby, W.M.M., sherries, J.C and Truck, M. 1966. "Antibiotic susceptibility testing by a standardized single disc method". Am. J. Clin. Pathol. 36 (3): 493-496